Advances in nucleic acid-based detection methods.
نویسندگان
چکیده
منابع مشابه
Advances in Nucleic Acid Detection and Quantification
The last decade has seen many changes in molecular biology at the bench, as we have moved away from a primary goal of cataloguing genes andmRNA towards techniques that detect and quantify nucleic acid molecules even within single cells. With the invention of the polymerase chain reaction (PCR), a nucleic acid sequence could now be amplified to generate a large number of identical copies, and th...
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© 2017 Annals of Cardiac Anaesthesia | Published by Wolters Kluwer Medknow The Editor, The primary determinant of survival in patients that develop sepsis is the time for recognition of infection and initiation of antibiotics.[1] The culture of an organism, still the gold standard, takes nearly 48–72 h to grow and test the antibiotic susceptibility of organism by conventional methods.[2] Nuclei...
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conclusions despite rapidity, automation, accuracy, cost-effectiveness, high sensitivity, and high specificity of molecular techniques, each type of molecular technology has its own advantages and disadvantages. results various types of molecular biology tools can be used to detect and quantify viral genomic elements and analyze the sequences. these molecular assays are proper technologies for ...
متن کاملDeveloping nucleic acid-based electrical detection systems
Development of nucleic acid-based detection systems is the main focus of many research groups and high technology companies. The enormous work done in this field is particularly due to the broad versatility and variety of these sensing devices. From optical to electrical systems, from label-dependent to label-free approaches, from single to multi-analyte and array formats, this wide range of po...
متن کاملAdvances in Single-molecule Nucleic Acid Sequencing
The ability to quickly and accurately obtain sequence information from single molecules of DNA and RNA has far-reaching implications for our understanding of biology. In the work presented here, we have made several advances in the area of single-molecule DNA and RNA sequencing. Specifically, in attempting to increase the read length of DNA polymerase, we have assayed several custom synthesized...
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ژورنال
عنوان ژورنال: Clinical Microbiology Reviews
سال: 1992
ISSN: 0893-8512,1098-6618
DOI: 10.1128/cmr.5.4.370-386.1992